ABO antibody product blog
Tags: Antibody; ABO; ABO antibody; Antigen H Type 2;
The ABO abo (Catalog #MBS439652) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Blood Group Antigen H Type 2 (CD173) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Blood Group Antigen H Type 2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin.Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the ABO abo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ABO abo product has the following accession number(s) (GI #58331216) (NCBI Accession #NP_065202.2) (Uniprot Accession #P16442). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Blood Group Antigen H Type 2 (CD173) Mouse Monoclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Blood Group Antigen H Type 2 Monoclonal Antibody (19-OLE))
This MAb preferably reacts with determinants of chain An and H type 3 (Gal 1-3GalNAc-R) and (Gal 1-3GalNAc-R), but not with type 1 and 2 chain structures. It is not reactive with immuno-dominant A trisaccharide. This MAb is applicable for tissue staining in tumor patients with blood groups An and AP. It shows a highly heterogeneous reactivity in human colon tumor tissue and adjacent mucosa. Blood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detect on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulationduring maligant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells and in secretion of certain indiviuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
Cellular Localization: Cell Surface
Immunogen: Mucin isolated from an ovarian cyst fluid. Hu-Chromosome Location: 9q34.2
Positive Control: KG1 cells or human colorectal carcinoma. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ABO are readily searchable from our website. Different antibodies against the same target such as ABO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.