anti-7-KC antibody product blog
Tags: Antibody; Monoclonal Antibody; 7-Ketocholesterol; anti-7-KC antibody; 7-KC;
The 7-KC n/a (Catalog #MBS808718) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 7-Ketocholesterol Antibody, Clone 7E1 reacts with Species Independent and may cross-react with other species as described in the data sheet. MyBioSource\'s 7-Ketocholesterol can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA).WB: 1:1000
ICC/IF: 1:50
ELISA: 1:1000. Researchers should empirically determine the suitability of the 7-KC n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the 7-Ketocholesterol Antibody, Clone 7E1 with the following immunoassay(s):
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Western Blot (WB) (Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 7E1. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: 7-ketocholesterol-BSA (0.5 ug). Lane 5: 7-ketocholesterol-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.)
Target: 7-Ketocholesterol
Immunogen: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate: Unconjugated
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing 7-KC are readily searchable from our website. Different antibodies against the same target such as 7-KC may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.