anti-Abcc8 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-ABCC8 antibody; ABCC8; SUR1;
The Abcc8 abcc8 (Catalog #MBS802683) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SUR1 Antibody: ATTO 488 reacts with Human, Mouse, Hamster, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SUR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF).WB (1:1000)
IHC (1:1000)
ICC/IF (1:100)
Optimal dilutions for assays should be determined by the user. Researchers should empirically determine the suitability of the Abcc8 abcc8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Abcc8 abcc8 product has the following accession number(s) (GI #148368981) (NCBI Accession #NP_037171.2) (Uniprot Accession #Q09429). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SUR1 Antibody: ATTO 488 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (MBS802683).
Lane 1: Molecular Weight Ladder.
Lane 2: Rat Brain Membrane.
Load: 15 �g.
Block: 2% BSA and 2% Skim Milk in 1X TBST.
Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (MBS802683) at 1:200 for 16 hours at 4�C.
Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT.
Color Development: ECL solution for 6 min in RT.
Predicted/Observed Size: ~160 kDa.)
Immunocytochemistry/Immunofluorescence(ICC/IF) ( Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (MBS802683).
Tissue: Neuroblastoma cell line (SK-N-BE).
Species: Human.
Fixation: 4% Formaldehyde for 15 min at RT.
Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (MBS802683) at 1:100 for 60 min at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT.
Localization: Cytoplasm, Nucleus.
Magnification: 60X.
(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SUR1 Antibody. (D) Composite.)
Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four Kir6.x and four SUR subunits form an ion conducting channel commonly referred to as the KATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir6.x potassium channel. Hence the KATP channel monitors the energy balance within the cell (2).