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anti-Abcc9 antibody product blog

Posted on 2015-09-04 01:02:25 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-ABCC9 antibody; ABCC9; SUR2A;
The Abcc9 abcc9 (Catalog #MBS804385) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SUR2A Antibody: PE/ATTO 594 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SUR2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC).
1:1000 WB. Researchers should empirically determine the suitability of the Abcc9 abcc9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Abcc9 abcc9 product has the following accession number(s) (GI #113722108) (NCBI Accession #NP_001038185.1) (Uniprot Accession #P70170). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SUR2A Antibody: PE/ATTO 594 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat Brain Membrane showing detection of ~120 kDa SUR2A protein using Mouse Anti-SUR2A Monoclonal Antibody, Clone S319A-14. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-SUR2A Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~120 kDa.)
Western Blot (WB) Abcc9.

Background Info: Detects ~120kDa. Does not crossreact with SUR2B.

Scientific Background: Sulfonylurea receptors (SUR) are membrane proteins which are the molecular targets of the sulfonylurea class of anti-diabetic drugs whose mechanism of action is to promote insulin release from pancreatic beta cells. More specifically, SUR proteins are subunits of the inward-rectifier potassium ion channels Kir6.x (6.1 and 6.2) (1). The association of four K ir6.x and four SUR subunits form an ion conducting channel commonly referred to as the K ATP channel. The primary function of the sulfonylurea receptor is to sense intracellular levels of the nucleotides ATP and ADP and in response facilitate the open or closing its associated Kir 6.x potassium channel. Hence the K ATP channel monitors the energy balance within the cell (2).

Conjugate: ATTO 594. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Abcc9 are readily searchable from our website. Different antibodies against the same target such as Abcc9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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