anti-ABCG5 antibody product blog
Tags: Antibody; Monoclonal Antibody; ABCG5; anti-ABCG5 antibody;
The ABCG5 abcg5 (Catalog #MBS476429) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ABCG5 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ABCG5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the ABCG5 abcg5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The ABCG5 abcg5 product has the following accession number(s) (GI #11967969) (NCBI Accession #NP_071881.1) (Uniprot Accession #Q9H222). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-ABCG5 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot analysis using ABCG5 mAb against human ABCG5 recombinant protein. (Expected MW is 32.7 kDa))
Western Blot (WB) (Western blot analysis using ABCG5 mAb against HEK293 (1) and ABCG5 (AA)
Western Blot (WB) (Western blot analysis using ABCG5 mouse mAb against HL7702 (1), RAJI (2) and Jurkat (3) cell lysate.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ABCG5 mouse mAb with DAB staining.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.)
Entrez Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia.
Immunogen: Purified recombinant fragment of human ABCG5 (AA: 306-367) expressed in E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ABCG5 are readily searchable from our website. Different antibodies against the same target such as ABCG5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.