anti-ACSL5 antibody product blog
Tags: Antibody; Monoclonal Antibody; ACSL5; anti-ACSL5 antibody;
The ACSL5 n/a (Catalog #MBS644332) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ACSL5 (Long-chain-fatty-acid-CoA Ligase 5, Long-chain acyl-CoA Synthetase 5, LACS 5, ACS5, FACL5, UNQ633/PRO1250) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ACSL5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the ACSL5 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:
PQPVLPLLDL NNQSVGIEGG ARKGVSQKNN DLTSCCFSDA KTMYEVFQRG LAVSDNGPCL GYRKPNQPYR WLSYKQVSDR AEYLGSCLLH KGYKSS.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ACSL5 (Long-chain-fatty-acid-CoA Ligase 5, Long-chain acyl-CoA Synthetase 5, LACS 5, ACS5, FACL5, UNQ633/PRO1250) with the following immunoassay(s):
Western Blot (WB) (Western Blot detection against Immunogen (36.67kD).)
Western Blot (WB) (Western Blot analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody.|Lane 1: ACSL5 transfected lysate (82.3kD).|Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
Immunoprecipitation (IP) (Immunoprecipitation of ACSL5 transfected lysate using ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 rabbit polyclonal antibody.)
Testing Data (Detection limit for recombinant GST tagged ACSL5 is ~0.1ng/ml as a capture antibody.)
Western Blot (WB) (ACSL5 monoclonal antibody, Western Blot analysis of ACSL5 expression in HepG2.)
ACSL5 is an isozyme of the long chain fatty acid coenzyme A ligase family. All isozymes of this family convert free long chain fatty acids into fatty acyl CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. ACSL5 is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid induced glioma cell growth. Three transcript variants encoding two different isoforms have been found.
Immunogen: Partial recombinant corresponding to aa91-187, from human ACSL5 (NP_057318, 51703) with GST tag. MW of the GST tag alone is 26kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ACSL5 are readily searchable from our website. Different antibodies against the same target such as ACSL5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.