anti-ACTB antibody product blog
Tags: Antibody; ACTB; Monoclonal Antibody; anti-ACTB antibody;
The ACTB actb (Catalog #MBS9135602) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HRP-conjugated ACTB Monoclonal Antibody reacts with Human, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa and may cross-react with other species as described in the data sheet. MyBioSource\'s ACTB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:10000 - 1:100000. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ACTB actb product has the following accession number(s) (GI #4501885) (NCBI Accession #NP_001092.1) (Uniprot Accession #P60709). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HRP-conjugated ACTB Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HRP-Conjugated beta-actin antibody at 1:50000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.)
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Immunogen: Recombinant protein of human beta-actin
Immunogen Species: Human. Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location: Cytoplasm, cytoskeleton
Positive Samples: HeLa, HepG2, MCF-7, Mouse liver, Mouse kidney, Mouse lung, Rat liver, Rat kidney, Rat lung. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ACTB are readily searchable from our website. Different antibodies against the same target such as ACTB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Bone, Brain, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Skin tissues are correlated with this protein. Brain Diseases, Carcinoma, Carcinoma, Hepatocellular, Disease Models, Animal, Kidney Diseases, Liver Diseases, Liver Neoplasms, Lung Diseases, Necrosis, Neoplasms are some of the diseases may be linked to HRP-conjugated ACTB Monoclonal Antibody.