anti-Actin, pan antibody product blog
Tags: Antibody; Monoclonal Antibody; Actin, pan; anti-Actin, pan antibody;
The Actin, pan n/a (Catalog #MBS633223) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Actin, pan (alpha, beta, gamma) (Cy3) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Actin, pan can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot; Immunofluorescence.Suitable for use in Immunofluorescence, ELISA and Western Blot.
Recommended Dilutions: Immunofluorescence: 1:50-1:200
ELISA: 1:10,000-1:50,000; using 50-200g control antigen/well
Western Blot: 1500-1:2,000 (Chemiluminescence). Researchers should empirically determine the suitability of the Actin, pan n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are ~90% conserved, except in the N-terminal 18aa (50-60%homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
Immunogen: Synthetic peptide corresponding to human 11-aa-MAPS (SGPSIVHRKCF) of actin.
Source Information: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Actin, pan are readily searchable from our website. Different antibodies against the same target such as Actin, pan may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.