anti-ADAM-10 antibody product blog
Tags: Antibody; Monoclonal Antibody; ADAM-10; anti-ADAM-10 antibody;
The ADAM-10 adam10 (Catalog #MBS690356) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse ADAM-10 reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s ADAM-10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS).1. Western blotting: Yes (1:500-1000). Researchers should empirically determine the suitability of the ADAM-10 adam10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ADAM-10 adam10 product has the following accession number(s) (GI #150378458) (NCBI Accession #NP_031425.2) (Uniprot Accession #O35598). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metallo-protease-like domain. Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Metturn expected for reprolysins, disintegrin like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis. It also processes the 26 kDa membrane anchored protumor necrosis factor alpha (TNFalpha) to the 17 kDa mature TNF alpha. It cleaves myelin basic protein and type IV collagen. ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell.
Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse recombinant protein of A Disintegrin and Metalloprotease-like Domain 10 ectodomain (ADAM10). Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ADAM-10 are readily searchable from our website. Different antibodies against the same target such as ADAM-10 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Bone Marrow, Brain, Embryonic Tissue, Eye, Heart, Liver, Lung, Prostate, Skin tissues are correlated with this protein.