anti-ADRA2C antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-ADRA2C antibody; ADRA2C; Alpha2C Adrenergic;
The ADRA2C adra2c (Catalog #MBS801276) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Alpha 2C Adrenergic Receptor Antibody: ATTO 594 reacts with Mouse, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Alpha2C Adrenergic can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).1:1000 (WB). Researchers should empirically determine the suitability of the ADRA2C adra2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ADRA2C adra2c product has the following accession number(s) (GI #71773208) (NCBI Accession #NP_000674.2) (Uniprot Accession #P18825). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Alpha 2C Adrenergic Receptor Antibody: ATTO 594 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Monkey COS cells transfected with HA-tagged Alpha-2C showing detection of ~50 kDa Alpha-2C Adrenergic Receptor protein using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-80. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Alpha-2C. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.)
Background Info: Detects 50kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.
Scientific Background: The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
Conjugate: ATTO 594. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ADRA2C are readily searchable from our website. Different antibodies against the same target such as ADRA2C may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.