anti-AFP antibody product blog
Tags: Antibody; AFP; Monoclonal Antibody; anti-AFP antibody;
The AFP afp (Catalog #MBS206604) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AFP antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s AFP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the AFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The AFP afp product has the following accession number(s) (GI #4501989) (NCBI Accession #NP_001125) (Uniprot Accession #P02771). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AFP antibody with the following immunoassay(s):
Western Blot (WB) (The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AFP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: Mouse liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: Raw264.7 cell lysate)
Immunofluorescence (IF) (ICC/IF analysis of AFP in HaLe cells. The cell was stained with ATGA0488 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
AFP, also known as alpha-fetoprotein, is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. This protein binds to copper, nickel, fatty acids and bilirubin. The main function of this is to prevent the virilization of female fetuses. As it does not bind estrogen, its function in humans is less clear.
Antigen Species: Human
Immunogen: Recombinant human AFP (19-609aa) purified from insect cell. Clone Note: Anti-human AFP mAb, clone AT73E10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AFP protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing AFP are readily searchable from our website. Different antibodies against the same target such as AFP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.