anti-AlCAM antibody product blog
Tags: Antibody; ALCAM; Monoclonal Antibody; anti-ALCAM antibody;
The AlCAM alcam (Catalog #MBS9602311) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AlCAM Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s AlCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA).ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000. Researchers should empirically determine the suitability of the AlCAM alcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AlCAM alcam product has the following accession number(s) (GI #343168770) (NCBI Accession #NP_001230209.1) (Uniprot Accession #Q13740). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the AlCAM Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using AlCAM mAb against human AlCAM recombinant protein. (Expected MW is 26.3 kDa))
Description: This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Function: Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).
Subunit Structure: Homodimer (PubMed:7760007, PubMed:16352806, PubMed:15048703, PubMed:26146185). Interacts (via extracellular domain) with CD6 (via extracellular domain) (PubMed:7760007, PubMed:8823162, PubMed:15048703, PubMed:16914752, PubMed:24945728, PubMed:26146185). Homodimerization and interaction with CD6 involve the same region and cannot occur simultaneously. The affinity for CD6 is much higher than the affinity for self-association (PubMed:15048703). Interacts (via glycosylated extracellular domain) with LGALS1 and LGALS3 (PubMed:24945728). Interaction with LGALS1 or LGALS3 inhibits interaction with CD6 (PubMed:24945728).
Post-translational Modifications: Glycosylated.
Similarity: The CD6 binding site is located in the N-terminal Ig-like domain.