anti-ALOX15 antibody product blog
Tags: Antibody; Monoclonal Antibody; ALOX15; anti-ALOX15 antibody;
The ALOX15 n/a (Catalog #MBS838702) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s ALOX15 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB).IF: 1:100
IHC: 1:150
WB: 1:500-2000. Researchers should empirically determine the suitability of the ALOX15 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ALOX15 antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALOX15 protein (Right) or empty vector (Left) detected with ALOX15 antibody)
Immunohistochemistry (IHC) (Immunohistochemical analysis of ALOX15 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using ALOX15 antibody)
Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ALOX15 protein using ALOX15 antibody)
Mouse monoclonal ALOX15 antibody.
Biological Significance: Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. There are six lipoxygenase isozymes; 5-LOX, 12R-LOX, 12S-LOX, 15-LOX-1 and 15-LOX-2, which catalyze the regio-selective addition of oxygen into arachidonic acid, and epidermal LOX (E-LOX) which metabolizes 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) to a specific epoxyalcohol compound. Different isozymes have different physiological roles; 5-, 12R-, 12S- and 15-LOX-1 have proliferative, antiapoptotic and angiogenic functions whilst 15-LOX-2 has roles in differentiation, growth arrest and the induction of apoptosis. E-LOX, the most divergent member of this family, is involved in terminal differentiation of keratinocytes and has a major role in skin barrier function. Cross-Reactivity: Human
Immunogen: ALOX15 antibody was raised in Mouse using full length human recombinant protein of human ALOX15 produced in HEK293T cell as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ALOX15 are readily searchable from our website. Different antibodies against the same target such as ALOX15 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.