|The ANXA1 anxa1 (Catalog #MBS2012545) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Annexin A1 (ANXA1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Annexin A1 (ANXA1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB).
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ANXA1 anxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ANXA1 anxa1 product has the following accession number(s) (GI #4502101) (NCBI Accession #NP_000691.1) (Uniprot Accession #P04083). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA LHKAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQETGK PLDETLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR DINRVYREEL KRDLAKDITS DTSGDFRNAL LSLAKGDRSE DFGVNEDLAD SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK VLDLELKGDI EKCLTAIVKC ATSKPAFFAE KLHQAMKGVG TRHKALIRIM.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Monoclonal Antibody to Annexin A1 (ANXA1) with the following immunoassay(s):
Western Blot (WB) Knockout Verification (Knockout Verification: Lane 1: Wild-type Hela cell lysate
Lane 2: ANXA1 knockout Hela cell lysate
Predicted MW: 39kDa ;Observed MW: 39kDa;Primary Ab: 2ug/ml Mouse Anti-Human ANXA1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody;)
Western Blot (WB) (Western Blot; Sample: Lane1: Human K562 Cells; Lane2: Human Hela Cells.)
Western Blot (WB) (Western Blot; Sample: Recombinant ANXA1, Human.)
Immunogen (Antigen): Recombinant ANXA1 (Met1~Asn346) expressed in E.coli. Immunogen (Antigen): Recombinant ANXA1 (Met1~Asn346) expressed in E.coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ANXA1 are readily searchable from our website. Different antibodies against the same target such as ANXA1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Carcinoma, Carcinoma, Squamous Cell, Fibrosis, Inflammation, Lymphatic Metastasis, Necrosis, Neoplasm Metastasis, Neoplasms, Pain are some of the diseases may be linked to Monoclonal Antibody to Annexin A1 (ANXA1).