anti-Arginylation antibody product blog
Tags: Antibody; Monoclonal Antibody; Arginylation; anti-Arginylation antibody;
The Arginylation n/a (Catalog #MBS808425) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The N-terminal Arginylation Antibody, Clone 11G1: HRP reacts with Species Independent and may cross-react with other species as described in the data sheet. MyBioSource\'s Arginylation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:1000. Researchers should empirically determine the suitability of the Arginylation n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the N-terminal Arginylation Antibody, Clone 11G1: HRP with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 11G1. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA, and REHKH-BSA.)
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
Target: N-terminal Arginylation
Immunogen: Synthetic N-terminal arginylated peptide conjugated to KLH
Cellular Localization: Endoplasmic Reticulum
Conjugate: HRP
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Arginylation are readily searchable from our website. Different antibodies against the same target such as Arginylation may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.