|The ARNT arnt (Catalog #MBS246679) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 3D10] (IgG2a,k) to Human ARNT / HIF-1-Beta reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ARNT / HIF-1-Beta can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA).
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein. Researchers should empirically determine the suitability of the ARNT arnt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ARNT arnt product has the following accession number(s) (GI #4502231) (NCBI Accession #NP_001659.1) (Uniprot Accession #P27540). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 3D10] (IgG2a,k) to Human ARNT / HIF-1-Beta with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-ARNT antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to ARNT on HeLa cell. [antibody concentration 10 ug/ml])
Western Blot (WB) (Western blot of ARNT expression in transfected 293T cell line.)
Western Blot (WB) (Western blot of ARNT expression in HeLa nuclear extract.)
ELISA (EIA) (Detection limit for recombinant GST tagged ARNT is approximately 0.03 ng/ml as a capture antibody.)
Transcriptional responses to many environmental and developmental stimuli are mediated by the basic helix-loop-helix (bHLH)-PAS family of proteins, which form homodimers that bind regulatory DNA sequences. ARNT/HIF-1 beta regulates the expression of different sets of genes in response to distinct environmental challenges. ARNT forms a complex with arylhydrocarbon receptor (AHR) to activate expression of genes encoding P450.
Target Species: Human
Immunogen Description: ARNT (AAH60838, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen: ARNT / HIF-1-Beta antibody was raised against aRNT (AAH60838, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ARNT are readily searchable from our website. Different antibodies against the same target such as ARNT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.