|The ASS ass1 (Catalog #MBS249325) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ASS1 / ASS Antibody (clone 2B10) IHC-plus reacts with Mouse, Rat, Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s ASS1 / ASS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS, ELISA (EIA).
Flow Cytometry (1:200 - 1:400)
ICC (1:200 - 1:1000)
IHC-P (10 ug/ml)
WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the ASS ass1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ASS ass1 product has the following accession number(s) (GI #53759107) (NCBI Accession #NP_000041.2) (Uniprot Accession #P00966). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-ASS1 / ASS Antibody (clone 2B10) IHC-plus with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-ASS1 / ASS antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunofluorescence (IF) (Immunofluorescence of HeLa cells using ASS1 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.)
Western Blot (WB) (Western blot using ASS1 monoclonal antibody against human ASS1 (AA: 40-236) recombinant protein. (Expected MW is 47 kDa))
Western Blot (WB) (Western blot using ASS1 mouse monoclonal antibody against A431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A549 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.)
ELISA (EIA) (Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
ASS1 Antibody, Argininosuccinate synthetase Antibody, ASS Antibody, Argininosuccinate synthase 1 Antibody, CTLN1 Antibody, Citrulline--aspartate ligase Antibody, Citrulline-aspartate ligase Antibody, Argininosuccinate synthase Antibody, Argininosuccinate synthetase 1 Antibody Description: Arginosuccinate synthetase catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 ASS pseudogenes scattered across the human genome; the ASS gene located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. The ASS primary transcript is subject to alternative splicing in the 5\' non-coding region; a splice variant lacking exon 2 occurs in liver and fibroblast cells.
Immunogen: ASS1 / ASS antibody was raised against purified recombinant fragment of human ASS1 expressed in E Coli.
Immunogen Description: Purified recombinant fragment of human ASS1 expressed in E Coli.
Immunogen Type: Recombinant protein. Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ASS are readily searchable from our website. Different antibodies against the same target such as ASS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.