anti-AT5G64690 antibody product blog
Tags: Antibody; Monoclonal Antibody; NEUROFILAMENT H; anti-AT5G64690 antibody; AT5G64690;
The AT5G64690 n/a (Catalog #MBS6012857) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament H, phosphorylated (Neurofilament Heavy Polypeptide, NF-H, Neurofilament Triplet H Protein, 200kD Neurofilament Protein, NEFH, NFH) reacts with Human, Porcine, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Neurofilament H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.
Dilution: Immunofluorescence: 1:1000
Immunohistochemistry: Formalin-fixed sections and frozen sections in tissue culture.
Western Blot: 1:10,000. Researchers should empirically determine the suitability of the AT5G64690 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The AT5G64690 n/a product has the following accession number(s) (GI #15237758) (NCBI Accession #NP_201274.1) (Uniprot Accession #Q9FGG4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Neurofilament H, phosphorylated (Neurofilament Heavy Polypeptide, NF-H, Neurofilament Triplet H Protein, 200kD Neurofilament Protein, NEFH, NFH) with the following immunoassay(s):
Testing Data (Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including MBS6012857 (lane 2).)
Immunohistochemistry (IHC) (Human cerebellar cortex fixed in formalin, embedded in paraffin and stained with MBS6012857 using the ABC (avidin biotin conjugate) method. The section was counterstained with heamatoxylin-eosin (blue). MBS6012857 stains prominent basket cell axons surrounding the large Purkinje neurons. Granule cell layer at bottom of image, molecular layer at top.)
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (HUGO names are NEFL, NEFM and NEFH respectively). NF-H is high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220kD, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig\'s disease) and Alzheimer\'s disease. 031397 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with 031397 in dendritic and perikaryal neurofilaments. The HGNC name for this protein is NEFH.