anti-ATP7B antibody product blog
Tags: Antibody; Monoclonal Antibody; ATP7B; Copper-Transporting ATPase2; anti-ATP7B antibody;
The ATP7B atp7b (Catalog #MBS802469) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Copper Transporting ATPase 2 Antibody: Biotin reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Copper-Transporting ATPase2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP).1:1000 (WB). Researchers should empirically determine the suitability of the ATP7B atp7b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ATP7B atp7b product has the following accession number(s) (GI #55743071) (NCBI Accession #NP_000044.2) (Uniprot Accession #B7ZLR4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Copper Transporting ATPase 2 Antibody: Biotin with the following immunoassay(s):
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29. Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X.)
Western Blot (WB) (Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody, Clone S62-29. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Copper Transporting ATPase 2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.)
Background Info: Detects ~160kDa in rat brain membrane preparations
Scientific Background: The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from the cell. ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; An is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson\'s disease, an inherited disorder causing copper poisoning in the brain and liver, characterized by neurological symptoms and hepatic damage.