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anti-Atxn1 antibody product blog

Posted on 2015-12-04 18:21:03 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; ATXN1; anti-ATXN1 antibody; Ataxin 1;
The Atxn1 atxn1 (Catalog #MBS803830) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ataxin 1 Antibody: PerCP reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Ataxin 1 can be used in a range of immunoassay formats including, but not limited to, Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP).
1:1000 WB. Researchers should empirically determine the suitability of the Atxn1 atxn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Atxn1 atxn1 product has the following accession number(s) (GI #334358889) (NCBI Accession #NP_001186233.1) (Uniprot Accession #P54254). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Ataxin 1 Antibody: PerCP with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S76-8. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~85 kDa.)
Western Blot (WB) Atxn1.

Background Info: Detects a 85 kDa protein.

Scientific Background: Ataxin-1 is a member of the ATXN1 protein family and contains a single AXH domain. It is a neurodegenerative disorder protein thought to have a role in the metabolism of RNA as it has been shown to localize to the RNA and transcription dependent inclusions within the nucleus. A mutation of Ataxin-1 is the cause of spinocerebellar ataxia type-1 (SCA1), a progressive, neurodegenerative disease that is autosomal dominant and primarily affects the Purjinke cells found in brain stem neuronal populations and the cerebellum. Expression of Ataxin-1 is almost ubiquitous, except in the brain where it is isolated to populations of neurons.

Conjugate: PerCP. Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Atxn1 are readily searchable from our website. Different antibodies against the same target such as Atxn1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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