anti-BAX antibody product blog
Tags: Antibody; anti-Bax antibody; Bax; Monoclonal Antibody;
The Bax bax (Catalog #MBS475223) is an Antibody produced from MouseSource: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bax Mouse mAb reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s Bax can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
WB: 1:1000. Researchers should empirically determine the suitability of the Bax bax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Bax bax product has the following accession number(s) (GI #612149787) (NCBI Accession #NP_001278357.1) (Uniprot Accession #Q07812). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Bax Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of Bax in COS7, Raji, CEM and Hela cell lysates using Bax mouse mAb (1:1000 diluted). Predicted band size:20KDa. Observed band size:20KDa.)
Entrez Summary: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. (provided by RefSeq, Jul 2008).
Immunogen: Purified recombinant human Bax protein fragments expressed in E Coli
Sensitivity: This antibody detects endogenous levels of Bax and does not cross-react with related proteins.
Sequence Similarities: Belongs to the Bcl-2 family. Subunit Structure: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X (L) BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X (L) Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with human cytomegalovirus/HHV-5 protein vMIA/UL37. Interacts with BOP/C22orf29.
Subcellular Location: Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm.