anti-BCL2L11 antibody product blog
Tags: Antibody; Monoclonal Antibody; BCL2L11; anti-BCL2L11 antibody; BimS, EL, L;
The BCL2L11 bcl2l11 (Catalog #MBS603522) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The BimS, EL, L (BOD, Bcl2l11, BCL2-like 11 Apoptosis Facilitator, Bcl2 Interacting Mediator of Cell Death) reacts with Human, Monkey, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s BimS, EL, L can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS).Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot.
Dilution: Immunohistochemistry: only EM
Immunoprecipitation: 2ug/ml
Western Blot: 1-5ug/ml
Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the BCL2L11 bcl2l11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BCL2L11 bcl2l11 product has the following accession number(s) (GI #18202042) (NCBI Accession #O43521.1) (Uniprot Accession #O43521). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BimS, EL, L (BOD, Bcl2l11, BCL2-like 11 Apoptosis Facilitator, Bcl2 Interacting Mediator of Cell Death) with the following immunoassay(s):
Testing Data (Figure A: Hybridomas producing MAbs to Bim (peptide) were identified using clones of the IL-3-dependent mouse myeloid cell line FDC-P1 stably expressing Bcl-2 alone, or Bcl-2 plus mouse BimL mixed at a 1:1 ratio. Transfected cells were fixed, permeabilized and stained with the MBS603522, the mouse anti-Glu-Glu (EE) MAb (positive control) or with secondary antibody alone (negative control). Staining was visualised by FITC-conjugated goat anti-rat or anti-mouse IgG antibodies.)
Western Blot (WB) (Figure B: Western blotting with anti-Bim MAb recognizes all known isforms of Bim. Western blotting of lysates of 293T cells cells transfected with EE-tagged forms of mouse or HA tagged forms of human Bim. Blots were probed with MBS603522, which detected EE mouse BimS, BimL, BimEL and EE human, BimSS, BimS, BimLS, BimL, BimELS, BimEL. Probing with an anti-Hsp-70 MAb was used as a loading control for all blots.)
Western Blot (WB) (Figure C: Anti-Bim MAb detects endogenous Bim expression in mouse tissues but not those from Bim knockout mice. Bim protein was revealed in lysates from mouse tissues (5 x 106/lane) by Western blotting withMBS603522 and detection by ECL. Probing with an anti-Hsp-70 MAb was used as a loading control.)
Testing Data
Immunogen: Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.