anti-beta Tubulin antibody product blog
Tags: Antibody; Monoclonal Antibody; beta Tubulin; anti-beta Tubulin antibody;
The beta Tubulin n/a (Catalog #MBS533153) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The beta Tubulin antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s beta Tubulin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB).IF: 1:250-1:1,000
WB: 1:250-1:1,000. Researchers should empirically determine the suitability of the beta Tubulin n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The beta Tubulin n/a product has the following accession number(s) (GI #166496) (NCBI Accession #AAA32689.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the beta Tubulin antibody with the following immunoassay(s):
Western Blot (WB) (The extracts of Mouse brain (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human beta-Tubulin (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse monoclonal beta Tubulin antibody.
Source Note: Anti-human beta-Tubulin antibody, clone AT5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human beta-Tubulin protein.
Biological Significance: Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Cross-Reactivity: Human, Mouse
Immunogen: beta Tubulin antibody was raised in mouse using recombinant human beta-Tubulin (1-445aa) purified from E Coli as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing beta Tubulin are readily searchable from our website. Different antibodies against the same target such as beta Tubulin may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.