anti-BHMT antibody product blog
Tags: Antibody; Monoclonal Antibody; BHMT; anti-BHMT antibody;
The BHMT bhmt (Catalog #MBS249430) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-BHMT Antibody (clone 3E11) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s BHMT can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS).Flow Cytometry (1:100)
IF (1:100)
IHC-P (10 ug/ml)
WB (1:500). Researchers should empirically determine the suitability of the BHMT bhmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BHMT bhmt product has the following accession number(s) (GI #157266337) (NCBI Accession #NP_001704.2) (Uniprot Accession #Q93088). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-BHMT Antibody (clone 3E11) IHC-plus with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunofluorescence (IF) (Anti-BHMT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.)
Western Blot (WB) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.)
Flow Cytometry (FC/FACS) (HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.)
BHMT Antibody, BHMT1 Antibody Description: Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.
Immunogen: BHMT antibody was raised against full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
Immunogen Description: Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
Immunogen Type: Recombinant protein. Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BHMT are readily searchable from our website. Different antibodies against the same target such as BHMT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.