anti-BMP4 antibody product blog
Tags: Antibody; BMP4; Monoclonal Antibody; anti-BMP4 antibody;
The BMP4 bmp4 (Catalog #MBS9602822) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BMP4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s BMP4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).ELISA: 1:10000
WB: 1:500-1:2000. Researchers should empirically determine the suitability of the BMP4 bmp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BMP4 bmp4 product has the following accession number(s) (GI #157276593) (NCBI Accession #NP_001193.2) (Uniprot Accession #P12644). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BMP4 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using BMP4 mouse mAb against BMP4-hIgGFc transfected HEK293 cell lysate.)
Description: The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors.BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix.
Function: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).
Subunit Structure: Homodimer; disulfide-linked (By similarity). Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with SOSTDC1. Interacts with FBN1 (via N-terminal domain) and FBN2 (PubMed:18339631).
Similarity: Belongs to the TGF-beta family.
Immunogen: Purified recombinant fragment of human BMP4 expressed in E. Coli
Subcellular Location: Secreted > Extracellular Space > Extracellular Matrix.
Tissue Specificity: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.