anti-BoNT B, lightchain antibody product blog
Tags: Antibody; Monoclonal Antibody; BoNT B, lightchain; anti-BoNT B, lightchain antibody;
The BoNT B, lightchain n/a (Catalog #MBS468003) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-botulinum neurotoxin type B (BoNT/B) monoclonal antibody reacts with Clostridium Botulinum and may cross-react with other species as described in the data sheet. MyBioSource\'s BoNT B, lightchain can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA.Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the BoNT B, lightchain n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-botulinum neurotoxin type B (BoNT/B) monoclonal antibody with the following immunoassay(s):
Testing Data (Fig.1: FACS analysis of BOSC23 cells using GR-3G7 Cat.# GM-0701. BOSC23 cells were transiently transfected with an expression vector encoding either BoNT/B (red curve) or an irrelevant protein (control transfectant). Binding of GR-3G7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with BoNT/B transfected cells.)
Testing Data #2 (Fig.2: BOSC23 cells were transiently transfected with expression vectors containing the cDNA of the light chain of botulinum toxin A-F. Expression of the constructs was tested with an anti-myc antibody (green curves). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified GR-3G7 was tested on all botulinum toxin transfectants. A positive signal was obtained only with BoNT/B transfected cells (red curves).)
Testing Data #3 (SDS-PAGE analysis of GR-3G7 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.3). Fig.3: SDS-PAGE analysis of purified GR- 3G7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GR-3G7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Background: Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C1,D,E,F, and G (1). BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release (2,3). BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment (4).
Buffer: phosphate buffered saline, pH 7.2
Immunogen: genetic immunisation with cDNA encoding BoNT/B
Selection: based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen: cDNA.