anti-BRSK2 antibody product blog
Tags: Antibody; Monoclonal Antibody; BRSK2; anti-BRSK2 antibody;
The BRSK2 n/a (Catalog #MBS6011882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRSK2, NT (BR Serine/threonine Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, STK29, HUSSY-12) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s BRSK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the BRSK2 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BRSK2 n/a product has the following accession number(s) (GI #117616926) (NCBI Accession #ABK42481.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BRSK2, NT (BR Serine/threonine Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, STK29, HUSSY-12) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from mouse and rat brain using MBS6011882.)
Brain-specific kinase 1 (BRSK1; SAD-B) and Brain-specific kinase 2 (BRSK2; SAD-A) are serine/threonine kinases closely related to AMPK. LKB1 phosphorylates Thr189 in the T-loop of BRSK1 and Thr174 in the T-loop of BRSK2, resulting in activation of the kinases (1). BRSK1 localizes to synaptic vesicles in the hippocampus and cerebellum, together with the active zone proteins Bassoon and CAST, and BRSK1 phoshorylates the active zone protein RIM1 (2). An alternatively spliced from of BRSK1 displays unique activity during the cell cycle, phosphorylating Ser131 of -tubulin and controling centrosome duplication (3). Neuronal polarization, including axon formation, is fundamental for normal brain development. BRSK1 -/- and BRSK2 -/- mice have defects in neuronal polarity and impaired corticogenesis (4). Knockdown of BRSK1 and BRSK2 in vitro diminishes axonal growth (5).
Immunogen: Synthetic peptide corresponding to residues near the amino terminus of human BRSK2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BRSK2 are readily searchable from our website. Different antibodies against the same target such as BRSK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.