anti-BTK antibody product blog
Tags: Antibody; anti-BTK antibody; BTK; Monoclonal Antibody;
The BTK btk (Catalog #MBS850806) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BTK Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s BTK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA).WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000. Researchers should empirically determine the suitability of the BTK btk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BTK btk product has the following accession number(s) (GI #4557377) (NCBI Accession #NP_000052.1) (Uniprot Accession #Q06187). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BTK Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1. Western blot analysis using BTK mouse mAb against K562 (1), MCF-7 (2), Jurkat (3) and HEK293 (4) cell lysate.)
Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human lymph-node tissues (left) and human lymph follicle tissues (right), showing cytoplasmic and membrane localization using BTK mouse mAb with DAB staining.)
Immunofluorescence (IF) (Figure 3. Immunofluorescence analysis of Jurkat cells using BTK mouse mAb.)
Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3, 4, 5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
Immunogen: Purified recombinant fragment of BTK expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BTK are readily searchable from our website. Different antibodies against the same target such as BTK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.