anti-BTK antibody product blog
Tags: Antibody; anti-BTK antibody; BTK; Monoclonal Antibody;
The BTK btk (Catalog #MBS9215577) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BTK Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s BTK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).Western Blot (WB) ~~ 1:1000
Immunoprecipitation (IP) ~~ 1:500. Researchers should empirically determine the suitability of the BTK btk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BTK btk product has the following accession number(s) (GI #4557377) (NCBI Accession #NP_000052.1) (Uniprot Accession #Q06187). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BTK Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot detection of BTK in Daudi,Jurkat(BTK negative),K562,Ramos and Raji cell lysates using BTK mouse mAb (1:1000 diluted).Predicted band size:77KDa.Observed band size:77KDa.)
Immunoprecipitation (IP) (Immunoprecipitation analysis of Raji cell lysates using BTK mouse mAb.)
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.