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anti-BUB1B antibody product blog

Posted on 2016-03-05 07:48:50 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; BUB1B; anti-BUB1B antibody;
The BUB1B bub1b (Catalog #MBS533292) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BUB1B antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s BUB1B can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB).
IF: 1:100
IHC: 1:50
WB: 1:2000. Researchers should empirically determine the suitability of the BUB1B bub1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The BUB1B bub1b product has the following accession number(s) (GI #294992075) (NCBI Accession #ADF57218.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the BUB1B antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BUB1B protein (Right) or empty vector (Left) detected with BUB1B antibody)
Western Blot (WB) BUB1B.

Immunohistochemistry (IHC) (Immunohistochemical analysis of BUB1B protein in paraffin embedded Adenocarcinoma of Human colon tissue using BUB1B antibody)
Immunohistochemistry (IHC) BUB1B.

Mouse monoclonal BUB1B antibody.

Biological Significance: BUB1B is a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. Immunogen: BUB1B antibody was raised in mouse using a protein expressed in 293T cell transfected with human BUB1B expression vector as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BUB1B are readily searchable from our website. Different antibodies against the same target such as BUB1B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Bone Marrow, Brain, Connective Tissue, Intestine, Liver, Lung, Muscle, Skin tissues are correlated with this protein. The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Carcinoma, Cell Transformation, Neoplastic, Colorectal Neoplasms, Disease Models, Animal, Microcephaly, Mosaic variegated aneuploidy syndrome, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to BUB1B antibody.
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