anti-C3/C3a antibody product blog
Tags: Antibody; Monoclonal Antibody; C3/C3a; anti-C3/C3a antibody;
The C3/C3a n/a (Catalog #MBS584407) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The C3/C3a, Human, mAb 474 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s C3/C3a can be used in a range of immunoassay formats including, but not limited to, Immunoassay (IA), Western Blot (WB).Application Notes: W: diabetic serum proteins were separated on PAGE-gel and transferred to nitrocellulose
Application Use: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting. Researchers should empirically determine the suitability of the C3/C3a n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Product Description: Monoclonal antibody 474 reacts with an epitope on C3a. The antibody reacts both with intact C3 as with C3a. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. During complement activation, C3 is proteolytically cleaved resulting in release of the anaphylatoxic peptide C3a. C3a is a small polypeptide consisting of 74 amino acids (ca.10kDa). C3a itself is very short-lived and in serum cleaved rapidly into the more stable C3a-desArg (also called acylation stimulating protein, ASP). C3a exerts its function through a specific receptor (C3aR), which belongs to the G-protein coupled receptor family. Expression of C3aR- has been reported in many cell types including myeloid and non-myeloid cells. Expression of C3aR on haematopoietic stem/progenitor cells has been shown to promote the trafficking/homing of these cells to the BM. Engagement of C3aR on DCs and T cells has been shown to up-regulate these cell functions.
C3a is a mediator of local inflammatory processes. It induces smooth muscle contraction, increases vascular permeability, and causes histamine release from mast cells and basophilic leukocytes. C3a is involved in inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and a variety of autoimmune diseases. An inherited deficiency of C3 predisposes the person to frequent assaults of bacterial infections. In ulcerative colitis, and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported.
Immunogen: C3a. Positive Control: Human serum. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing C3/C3a are readily searchable from our website. Different antibodies against the same target such as C3/C3a may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.