anti-C3/C3b antibody product blog
Tags: Antibody; Monoclonal Antibody; C3/C3b; anti-C3/C3b antibody;
The C3/C3b n/a (Catalog #MBS584234) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The C3/C3b, Human, mAb 755 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s C3/C3b can be used in a range of immunoassay formats including, but not limited to, Immunoassay (IA), Western Blot (WB).Application Notes: W: A non-reduced or reduced sample treatment and SDS-PAGE was used. The band size (s) are ~190 and ~100 kDa under non-reducing and reducing conditions respectively. (Ref.1)
Application Use: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the C3/C3b n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Product Description: The monoclonal antibody 755 recognizes an epitope located in the C-terminal 360 amino acids-on the alpha chain of C3, thereby recognizing C3b and full C3. The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes a person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody-mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. After activation of the complement system, certain enzymes become active, resulting in the cleavage of C3 into C3b and the anaphylatoxin C3a. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Within the alternative pathway of complement, C3b plays a critical role in the amplification loop initiated by spontaneous hydrolysis of C3.
Immunogen: Native C3. Positive Control: Human serum. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing C3/C3b are readily searchable from our website. Different antibodies against the same target such as C3/C3b may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.