|The CA9 ca9 (Catalog #MBS649431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CA9, CT (Carbonic Anhydrase 9, Carbonate Dehydratase IX, Carbonic Anhydrase IX, CA-IX, CAIX, Membrane Antigen MN, P54/58N, Renal Cell Carcinoma-associated Antigen G250, RCC-associated Antigen G250, pMW1, G250, MN) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CA9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:100. Researchers should empirically determine the suitability of the CA9 ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CA9 ca9 product has the following accession number(s) (GI #169636420) (NCBI Accession #NP_001207.2) (Uniprot Accession #Q16790). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
CA9, CT (Carbonic Anhydrase 9, Carbonate Dehydratase IX, Carbonic Anhydrase IX, CA-IX, CAIX, Membrane Antigen MN, P54/58N, Renal Cell Carcinoma-associated Antigen G250, RCC-associated Antigen G250, pMW1, G250, MN)
Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CAs can be divided into 3 distinct classes (a, b, and y) that evolved independently and have no significant homology in sequence and overall folding. All functional CAs catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the a class (1,2). CA9 is a member of alpha class, a plasma membrane protein with the catalytic domain in the extracellular space. Its expression is restricted to very few normal tissues (mainly the gastrointestinal tract) (2). CA9 expression is strongly induced by hypoxia and down-regulated by the wildtype von Hippel-Lindau (VHL) tumor suppressor protein. CA9 expression is increased in many types of tumor, especially in solid hypoxic tumors with a poor responsiveness to the conventional radio-and/or chemo-therapies; CA9 is considered as a tumor hypoxia marker and a promising target for cancer therapeutic intervention (3-5).
Immunogen: Synthetic peptide corresponding to residues surrounding Asp361 of human CA9. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CA9 are readily searchable from our website. Different antibodies against the same target such as CA9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.