|The Cacnb2 cacnb2 (Catalog #MBS801309) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CaVbeta2 Antibody: Biotin reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Cavbeta2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC).
1ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF). Researchers should empirically determine the suitability of the Cacnb2 cacnb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Cacnb2 cacnb2 product has the following accession number(s) (GI #16758716) (NCBI Accession #NP_446303) (Uniprot Accession #Q8VGC3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CaVbeta2 Antibody: Biotin with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.)
Western Blot (WB) (Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.)
Background Info: Detects ~78 kDa. No cross reactivity against Cavbeta1, Cavbeta3, Cavbeta4
Scientific Background: Cav Beat subunits are involved in the transport of the pore forming alpha1 subunit to the plasma membrane (3). They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore-forming subunit to the target membrane (4, 5). They also determine the biophysical properties of the calcium channel (5).
Conjugate: Biotin. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Cacnb2 are readily searchable from our website. Different antibodies against the same target such as Cacnb2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.