anti-Calcium/calmodulin-dependent protein kinase II antibody product blog
Tags: Antibody; Monoclonal Antibody; CaMKII; anti-Calcium/calmodulin-dependent protein kinase II antibody; Calcium/calmodulin-dependent protein kinase II;
The Camk2d camk2d (Catalog #MBS565021) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CaMKII monoclonal antibody (6G9) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s CaMKII can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB).Western Blot (Colorimetric, 1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~50-60kDa by Western blot. Researchers should empirically determine the suitability of the Camk2d camk2d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Camk2d camk2d product has the following accession number(s) (GI #125288) (NCBI Accession #P15791.1) (Uniprot Accession #P15791). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CaMKII monoclonal antibody (6G9) with the following immunoassay(s):
Western Blot (WB) (Western Blot Analysis of Calmodulin-Dependent Protein Kinase II: Lane 1: MWM, Lane 2: Mouse Brain, Lane 3: Rat Brain.)
CaMKIIa, the alpha subunit of Ca2+ calmodulin-dependent protein kinase II is part of a family of multifunctional protein kinases, which play a major role in Ca2+-mediated signal transduction. CaMKIIa is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites as well as the soma of the neuron. The neuronal CaMKII consists of two major subunits of 52 and 60 kDa which are encoded by a- and b-CaMKII genes, respectively. Additional isoforms are generated by alternative splicing of these as well as of the ubiquitious g- and d-CaMKII genes. Each subunit has an ATP-binding domain (arginine-X-X-serune/threonine), consensus phosphorylation site, catalytic domain, and a centrally located regulatory domain which has calmodulin binding activity. Activation and autophosphorylation of CaMKII may regulate numerous neuronal processes which includes two forms of synaptic plasticity, long term potentiation and long term depression. Neuronal CaMKII subunits assemble as large multimeric holoenzymes. The C-terminal association domains of 6-12 subunits assemble into a central globular structure from which the N-terminal catalytic/regulatory domains extend radially like petals of a flower. The subunit composition of the rat forebrain CaMKII holoenzyme consists of heteromers composed of an and beta subunits at a ratio of 2:1 and homomers composed of only a subunits. The association of CaMKII subunits leads to the positioning of their catalytic/regulatory domains in close proximity and the neighboring calmodulin-bound subunits cooperate to rapidly phosphorylate each other. Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.