anti-CASP9 antibody product blog
Tags: Antibody; Caspase-9; CASP9; Monoclonal Antibody; anti-CASP9 antibody;
The CASP9 casp9 (Catalog #MBS395038) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Caspase-9 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Caspase-9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CASP9 casp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CASP9 casp9 product has the following accession number(s) (GI #14790124) (NCBI Accession #NP_001220.2) (Uniprot Accession #P55211). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Caspase-9 with the following immunoassay(s):
Western Blot/Blotting (Western blot analysis using Caspase-9 antibody on MCF-7 cells negative (-) and positive (+) for caspase-3 and showing the proenzyme form of caspase-9 and one of the cleavage products after treatment with thapsigargin for 48 hours.)
Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-9 is a member of the CED-3 family and bear high similarity to caspase-3. Procaspase-9 can be activated by either caspase-3 or granzyme B, although they cleave the proenzyme to different size subunits. Cleavage by granzyme B produces an active enzyme which is capable of cleaving PARP. Also, the ability of caspase-3 to activate caspase-9 seems to suggest that caspase-9 is further downstream of caspase-3 and may be involved in later changes in cells observed undergoing apoptosis.
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-9 protein and mouse myeloma cells. Positive Control: MCF-7 cell lines. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CASP9 are readily searchable from our website. Different antibodies against the same target such as CASP9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CASP9 also interacts with the following gene(s): AKT1, APAF1, BCL2L1, BIRC2, BIRC3, CASP1, CASP3, DCC, DIABLO, XIAP.