anti-CASP9 antibody product blog
Tags: Antibody; Caspase 9; CASP9; Monoclonal Antibody; anti-CASP9 antibody;
The CASP9 casp9 (Catalog #MBS8241580) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Caspase 9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Caspase 9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP).Western Blot: (1/1000 - 1/3000); Immunoprecipitation: (1/50 - 1/200). Researchers should empirically determine the suitability of the CASP9 casp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CASP9 casp9 product has the following accession number(s) (GI #28558771) (NCBI Accession #P55211.3) (Uniprot Accession #P55211). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Caspase 9 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Caspase 9 expression in Hela (A), Jurkat (B), HepG2 (C), mouse heart (D), rat heart (E) whole cell lysates.)
Immunofluorescence (IF) (Immunofluorescent analysis of Caspase 9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunoprecipitation (IP) (Immunoprecipitation of Caspase 9 from 0.5mg Hela whole cell extract lysate, using anti-Caspase 9 Antibody.)
Mouse monoclonal to Caspase 9.
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Caspase 9. The exact sequence is proprietary. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CASP9 are readily searchable from our website. Different antibodies against the same target such as CASP9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Prostate, Vascular tissues are correlated with this protein. Brain Diseases, Breast Neoplasms, Carcinoma, Hepatocellular, Inflammation, Liver Diseases, Liver Neoplasms, Lung Neoplasms, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to Anti-Caspase 9 Antibody.