anti-CCL-3 antibody product blog
Tags: Antibody; Monoclonal Antibody; CCL-3; anti-CCL-3 antibody;
The CCL-3 ccl3 (Catalog #MBS690693) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human CCL-3 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CCL-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Neutralization (N).Western Blot (WB) 1:500-1000
IHC 1:20-100
Neutralization: Yes. Researchers should empirically determine the suitability of the CCL-3 ccl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CCL-3 ccl3 product has the following accession number(s) (GI #4506843) (NCBI Accession #NP_002974.1) (Uniprot Accession #P10147). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The macrophage inflammatory proteins 1alpha and 1beta were originally copurified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP 1alpha. Mature MIP1alpha and MIP1beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP1 proteins are members of the beta (CC) subfamily of chemokines. Both MIP1alpha and MIP1beta are monocyte chemoattractants in vitro. Additionally, the MIP1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP1alpha and MIP1beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP1alpha has also been shown to attract B cells as well as eosinophils. MIP1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP1alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP1alpha has been identified as CCR1 and CCR5.
Preparation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant protein of CCL-3 (also called MIP-1a).
Reconstitution buffer: PBS(sterile)
Antigen: Recombinant human CCL-3. Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml.
Remarks: This antibody was selected for its ability to detect human CCL-3 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CCL-3 are readily searchable from our website. Different antibodies against the same target such as CCL-3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.