anti-CCND1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-CCND1 antibody; CCND1;
The CCND1 ccnd1 (Catalog #MBS9230999) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CCND1 reacts with Human, MousePredicted: Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CCND1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB: 1:2000. Researchers should empirically determine the suitability of the CCND1 ccnd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CCND1 ccnd1 product has the following accession number(s) (GI #16950655) (NCBI Accession #NP_444284.1) (Uniprot Accession #P24385). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CCND1 with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-CCND1 at dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: HT-1080 whole cell lysate
Lane 3: L929 whole cell lysate
Lane 4: NIH/3T3 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Antigen Type: Synthesized Peptide
Antigen Source: Human. Cellular Location: Nucleus. Cytoplasm. Membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CCND1 are readily searchable from our website. Different antibodies against the same target such as CCND1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.