anti-CCNH antibody product blog
Tags: Antibody; Monoclonal Antibody; CCNH; anti-CCNH antibody; Cyclin H;
The CCNH ccnh (Catalog #MBS477196) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin H Mouse mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Cyclin H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:1000. Researchers should empirically determine the suitability of the CCNH ccnh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CCNH ccnh product has the following accession number(s) (GI #4502623) (NCBI Accession #NP_001230.1) (Uniprot Accession #P51946). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Cyclin H Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of Cyclin H in Jurkat, K562, Hela and 3T3 cell lysates using Cyclin H Mouse mAb (1:1000 diluted). Predicted band size: 38KDa. Observed band size:38KDa.)
Entrez Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.
UniPort Summary Function: Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Immunogen: Purified recombinant human Cyclin H protein fragments expressed in E Coli.
Sensitivity: This antibody detects endogenous levels of Cyclin H and does not cross-react with related proteins.
Sequence Similarities: Belongs to the cyclin family. Cyclin C subfamily.