|The CD103 n/a (Catalog #MBS5309427) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The CD103 antibody (biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD103 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunhistochemistry (IHC).
FC/FACS: = 0.125 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD103 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD103 antibody (biotin) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of mouse splenocytes with 0.06 ug of Armenian Hamster IgG Isotype Control (biotin) (open histogram) or 0.06 ug of CD103 antibody (biotin) (filled histogram) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta 7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells. Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.
Conjugate: Biotin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD103 are readily searchable from our website. Different antibodies against the same target such as CD103 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.