anti-CD105 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD105; anti-CD105 antibody;
The CD105 n/a (Catalog #MBS8505552) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD105 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CD105 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Endoglin(CD105) a major glycoprotein of human vascular endothelium, is a type I integral membrane protein with a large extracellular region.a hydrophobic transmembrane region and a short cytoplasmic tail.There are two forms of endoglin(S-endoglin and L-endoglin) that differ in the length of their cytoplasmic tails.However, the isoforms may have similar functional activity. When overexpressed in fibroblasts.both form disulfide-linked homodimers via their extracellular doains. Endoglin is an accessory protein of multiple TGF-beta superfamily kinase receptor complexes loss of function mutaions in the human endoglin gene cause hereditary hemorrhagic telangiectasia, which is characterized by vascular malformations, Deletion of endoglin in mice leads to death due to defective vascular development.
Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1�RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10�);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD105 are readily searchable from our website. Different antibodies against the same target such as CD105 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.