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anti-CD115 antibody product blog

Posted on 2016-02-02 07:21:56 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD115; anti-CD115 antibody;
The CD115 csf1r (Catalog #MBS215327) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD115 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD115 csf1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD115 csf1r product has the following accession number(s) (GI #126723423) (NCBI Accession #NP_001032948.2) (Uniprot Accession #P09581). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD115:RPE with the following immunoassay(s):
Testing Data (Staining of the mouse J774 cell line with Rat anti Mouse CD115 : RPE)
Testing Data CD115.

Rat anti Mouse CD115 antibody, clone 604B5 2E11 recognizes the murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Rat anti Mouse CD115 antibody, clone 604B5 2E11 has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.

Reconstitution: Reconstitute with 1.0 ml distilled water
Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by ion exchange chromatography. Immunogen: RAW 264 cells
Fusion Partners: Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD115 are readily searchable from our website. Different antibodies against the same target such as CD115 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Bone, Bone Marrow, Brain, Joint, Kidney, Liver, Lung, Skin, Spleen tissues are correlated with this protein. Breast Neoplasms, Cardiovascular Diseases, Inflammation, Kidney Diseases, Leukemia, Myeloid, Acute, Lung Diseases, Lung Neoplasms, Lymphoma, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to RAT ANTI MOUSE CD115:RPE.
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