|The CD115 n/a (Catalog #MBS5305418) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat monoclonal CD115 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD115 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Functional Assay, Western Blot (WB).
FC: <= 0.125 ug/test. Researchers should empirically determine the suitability of the CD115 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rat monoclonal CD115 antibody with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of normal human peripheral blood cells with 0.5 ug of Rat IgG1 kappa Isotype Control (open histogram) or 0.5 ug of CD115 antibody (filled histogram) followed by Anti-Rat IgG (biotin) and Streptavidin (PE). Cells in the monocyte gate were used for analysis.)
Assay Information: A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10? to 10? cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD115 are readily searchable from our website. Different antibodies against the same target such as CD115 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.