|The CD11c n/a (Catalog #MBS666165) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD11c-R-PE reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s CD11c can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
1:50 (or use 1.6mul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD11c n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD11c-R-PE with the following immunoassay(s):
Information: Human CD11c (aX integrin) complexes with CD18 (beta2 integrin) to form the complement receptor type 4 (CR4) heterodimer which binds to fibrinogen and is involved with monocyte/granulocyte adhesion during inflammatory responses. CD11c expression is restricted to leukocytes mainly of myeloid lineage with highest expression on macrophages. Antibody 3.9 recognizes the integrin alpha X subunit (CD11c) of about 150 kd.
Performance: Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated 10 minutes with 20 mul human IgG at 250 mug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD11c/R-PE at a 1:50 dilution (20mug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 17% subset of the cells (from a myeloid + lymph gate) stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD11c antibody. Conjugation: PE
Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at approximately 1.0 mg/ml.
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human rheumatoid synovial fluid cells/monocytes. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD11c are readily searchable from our website. Different antibodies against the same target such as CD11c may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.