Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-CD11c antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-OCRL antibody blog
 • anti-EGF antibody blog
 • anti-SAA antibody blog
 • anti-Gabrb3 antibody blog
 • anti-SRC antibody blog
 • anti-CD205 antibody blog
 • anti-ACAN antibody blog
 • anti-CD22 antibody blog
 • anti-RALA antibody blog
 • anti-LCAT antibody blog
 • GPC3 antibody blog
 • anti-Splunc2 antibody blog
 • anti-UBE2Q2 antibody blog
 • anti-CSPG4 antibody blog
 • anti-HSD17B10 antibody blog
 • anti-CD20 antibody blog
 • anti-PDGFA antibody blog
 • anti-MCP3 antibody blog
 • anti-EGF antibody blog
 • anti-CD3 antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • embryonic ectoderm development (EED) Antibody
 • Tumor necrosis factor receptor superfamily member 10B protei... Recombinant Protein
 • Prolactin (PRL) Western Blot Control
 • Fascin (FSCN1) Antibody
 • Creatine Kinase MM (CK-MM) >95% Pure (CKM) Protein
 • Advanced glycation end products (AGEs) ELISA Kit
 • Cofilin 1 (Ser-3) (CFL1) Antibody
 • Ciliary neurotrophic factor (CNTF) Antibody
 • KCNJ11 Antibody
 • 17-keto-steroid (17-KS) ELISA Kit
 • Vimentin (VIM) ELISA Kit
 • Annexin A3 (ANXA3) Antibody
 • ELOVL family member 7, elongation of long chain fatty acids ... ELISA Kit
 • IL-33 (IL33) ELISA Kit
 downarrow more ...

anti-CD11c antibody product blog

Posted on 2016-01-27 18:36:17 by mybiosource_staff
Share it on ...     
Tags: Antibody; Monoclonal Antibody; CD11c; anti-CD11c antibody;
The CD11c n/a (Catalog #MBS666165) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD11c-R-PE reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s CD11c can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
1:50 (or use 1.6mul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD11c n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD11c-R-PE with the following immunoassay(s):
Testing Data
Testing Data CD11c.

Information: Human CD11c (aX integrin) complexes with CD18 (beta2 integrin) to form the complement receptor type 4 (CR4) heterodimer which binds to fibrinogen and is involved with monocyte/granulocyte adhesion during inflammatory responses. CD11c expression is restricted to leukocytes mainly of myeloid lineage with highest expression on macrophages. Antibody 3.9 recognizes the integrin alpha X subunit (CD11c) of about 150 kd.

Performance: Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated 10 minutes with 20 mul human IgG at 250 mug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD11c/R-PE at a 1:50 dilution (20mug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 17% subset of the cells (from a myeloid + lymph gate) stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD11c antibody. Conjugation: PE
Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at approximately 1.0 mg/ml.
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human rheumatoid synovial fluid cells/monocytes. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD11c are readily searchable from our website. Different antibodies against the same target such as CD11c may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions