anti-CD13 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD13; anti-CD13 antibody;
The CD13 anpep (Catalog #MBS219723) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD13 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunohistology Paraffin*.Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. See Bertilaccio et al. for details. Researchers should empirically determine the suitability of the CD13 anpep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD13 anpep product has the following accession number(s) (GI #225637487) (NCBI Accession #NP_032512.2) (Uniprot Accession #P97449). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD13:Low Endotoxin with the following immunoassay(s):
Testing Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 (MBS212549), green in An and Rat anti Mouse CD8, clone YTS105.18, red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 647 (MBS212549A647))
Testing Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 (MBS212549) followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). High power)
Testing Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 (MBS212549) followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). Low power)
Testing Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 (MBS212549), green in An and Rat anti Mouse CD8, clone YTS105.18, red in B. Cis the merged image with nuclei counterstained blue using DAPI. Low power)
Testing Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 488 (MBS212549A488))
Testing Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 (MBS212549), green in An and Rat anti Mouse CD8, clone YTS105.18, red in B. Cis the merged image with nuclei counterstained blue using DAPI. Medium power)
Testing Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 (MBS212549) followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). Medium power)
Rat anti Mouse CD13 antibody, clone R3-63 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kDa subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. Rat anti Mouse CD13 antibody, clone R3-63 has been reported to block mouse APN enzyme activity (Kamoun et al. 2009).