|The CD13 n/a (Catalog #MBS666330) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD13-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD13 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FPS. Researchers should empirically determine the suitability of the CD13 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD13-Biotin with the following immunoassay(s):
Information: Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role. Antibody 22A5 recognizes the cell surface aminopeptidase-N enzyme.
Performance: Five x 10^5 cultured THP-1 cells were washed and pre incubated ~5 minutes with 20ul of 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD13/Biotin at 10 ug/ml. Cells were washed twice and incubated with 50 ul of 2 degree reagent Streptaviden/R-PE (MBS666783) after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.19 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control (MBS666441) at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD13 antibody (MBS666228). Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human osteosarcoma tissue. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD13 are readily searchable from our website. Different antibodies against the same target such as CD13 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.