Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-CD13 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-HDAC1 antibody blog
 • anti-RecA antibody blog
 • anti-Taf15 antibody blog
 • anti-Heartworm, Canine antibody blog
 • anti-FKBP5 antibody blog
 • anti-EGFR antibody blog
 • anti-ANK antibody blog
 • anti-Brain Natriuretic Peptide, aa1-32 antibody blog
 • anti-CRP antibody blog
 • anti-HSPA1L antibody blog
 • anti-CD4 antibody blog
 • SBA Clonotyping System-B6/C57J antibody panel blog
 • anti-CMTM5 antibody blog
 • anti-MICAL1 antibody blog
 • anti-CP4EPSPS mAb1 antibody blog
 • anti-NUCB1 antibody blog
 • anti-SYN1 antibody blog
 • anti-CK8 antibody blog
 • anti-Influenza A, Avian, H5N1, Hemagglutinin (HA) (Bird Flu) antibody blog
 • anti-IL28A antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • alpha Lactalbumin (LALBA) Antibody
 • Gliadin IgM ELISA Kit
 • Glutathione (GT) ELISA Kit
 • CHOP Antibody
 • Leptin ELISA Kit
 • DTW domain-containing protein 1 Antibody
 • superoxide dismutase 3, extracellular (SOD3) Antibody
 • fibulin 5 (FBLN5) Antibody
 • Macrophage Inflammatory Protein 2 (MIP-2) ELISA Kit
 • coxsackievirus A16 (CVA16) antibody (IgM) ELISA Kit
 • NKX3-1 Antibody
 • HERV-V_19q13.41 provirus ancestral Env polyprotein 1 (ERVV-1... ELISA Kit
 downarrow more ...

anti-CD13 antibody product blog

Posted on 2016-02-06 07:56:28 by mybiosource_staff
Share it on ...     
Tags: Antibody; Monoclonal Antibody; CD13; anti-CD13 antibody;
The CD13 n/a (Catalog #MBS666330) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD13-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD13 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FPS. Researchers should empirically determine the suitability of the CD13 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD13-Biotin with the following immunoassay(s):
Testing Data
Testing Data CD13.

Information: Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role. Antibody 22A5 recognizes the cell surface aminopeptidase-N enzyme.

Performance: Five x 10^5 cultured THP-1 cells were washed and pre incubated ~5 minutes with 20ul of 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD13/Biotin at 10 ug/ml. Cells were washed twice and incubated with 50 ul of 2 degree reagent Streptaviden/R-PE (MBS666783) after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.19 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control (MBS666441) at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD13 antibody (MBS666228). Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human osteosarcoma tissue. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD13 are readily searchable from our website. Different antibodies against the same target such as CD13 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions