Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-CD13 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-CD44 antibody blog
 • anti-Calicivirus antibody blog
 • anti-HLA-B antibody blog
 • anti-HCGalpha antibody blog
 • anti-E. histolytica antibody blog
 • anti-Tie2/CD202b/TEK antibody blog
 • anti-CD5 antibody blog
 • anti-FLOT1 antibody blog
 • anti-F2 antibody blog
 • anti-UCHL3 antibody blog
 • anti-ROR antibody blog
 • anti-HAGHL antibody blog
 • anti-GTF2I / TFII I antibody blog
 • anti-REEP1/2 antibody blog
 • anti-Stat92E antibody blog
 • anti-GSTA1 antibody blog
 • anti-AKT2 antibody blog
 • anti-PYCRL antibody blog
 • anti-AFP antibody blog
 • anti-Smad3 antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • IP-10 Antibody
 • VARICELLA ZOSTER gE Recombinant Protein
 • agouti-related protein (AGRP) ELISA Kit
 • Glutathione Peroxidase 1 (GPX1) ELISA Kit
 • ABCD1 Antibody
 • Cytosolic Phospholipase A2 (CPLA2) ELISA Kit
 • SRD5A2 Antibody
 • Bacillus cereus Phospholipase C Antibody
 • B-raf (BRAF) Positive Control
 • Ubiquitin (UBA52) Recombinant Protein
 • interferon, gamma (IFNG) ELISA Kit
 • IL1RA Antibody
 • HSC70 (HSP73) (HSPA8) Antibody
 • CCR6 Antibody
 downarrow more ...
separator

anti-CD13 antibody product blog

separator
Posted on 2016-02-06 07:56:28 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Monoclonal Antibody; CD13; anti-CD13 antibody;
separator
The CD13 n/a (Catalog #MBS666330) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD13-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD13 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FPS. Researchers should empirically determine the suitability of the CD13 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD13-Biotin with the following immunoassay(s):
Testing Data
Testing Data CD13.

Information: Human CD13 is a zinc-binding aminopeptidase-N enzyme expressed on the surface of myeloid cells. IL-4 will upregulate expression of CD13 which may play an anti-inflammatory role. Antibody 22A5 recognizes the cell surface aminopeptidase-N enzyme.

Performance: Five x 10^5 cultured THP-1 cells were washed and pre incubated ~5 minutes with 20ul of 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD13/Biotin at 10 ug/ml. Cells were washed twice and incubated with 50 ul of 2 degree reagent Streptaviden/R-PE (MBS666783) after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.19 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control (MBS666441) at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD13 antibody (MBS666228). Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human osteosarcoma tissue. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD13 are readily searchable from our website. Different antibodies against the same target such as CD13 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions