anti-CD134 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD134; anti-CD134 antibody;
The CD134 tnfrsf4 (Catalog #MBS213601) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD134 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD134 tnfrsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD134 tnfrsf4 product has the following accession number(s) (GI #6755909) (NCBI Accession #NP_035789.1) (Uniprot Accession #P47741). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD134:RPE with the following immunoassay(s):
Testing Data (Staining of stimulated rat spleen cells with Rat anti Mouse CD134:Azide Free (MBS210149))
Testing Data (Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134:Alexa Fluor 647 (MBS212139A647))
Testing Data (Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134:RPE)
Testing Data (Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134 (MBS212139))
Rat anti Mouse CD134 antibody, clone OX-86 recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin An activated mouse splenocytes.
Reconstitution: Reconstitute with 1ml distilled water
Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: CHO cells transfected with murine OX40
Fusion Partners: Spleen cells from immunised AO rats were fused with cells of the mouse NS1 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD134 are readily searchable from our website. Different antibodies against the same target such as CD134 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.