anti-CD137 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD137; anti-CD137 antibody;
The CD137 tnfrsf9 (Catalog #MBS666885) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD137 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the CD137 tnfrsf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CD137 tnfrsf9 product has the following accession number(s) (GI #163866805) (NCBI Accession #ABY47575.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Human CD137 with the following immunoassay(s):
Flow Cytometry (FC/FACS)
Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3). CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2). CD137 appears to be important for T cell proliferation and survival (4) and induces monocyte activation (5).
Immunogen: Ectodomain of human 4-1BB fusion protein s
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added. Performance: Ficoll prepared human peripheral blood lymphocytes were stimulated overnight in culture with 10 ng/ml PMA and 1mM Ionomicin. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD137 F(ab\')2 at 5 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.75 log10 fluorescent units when compared to a Mouse IgG1 F(ab\')2 negative control. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD137 are readily searchable from our website. Different antibodies against the same target such as CD137 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.