|The CD137L n/a (Catalog #MBS666045) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD137L-Purified (with Antibiotics) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD137L can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the CD137L n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD137L-Purified (with Antibiotics) with the following immunoassay(s):
Information: Human CD137 Ligand is a type II transmembrane protein constitutively expressed by monocytes, B cells neuroblastoma cells (1), and some activated T cells. The interaction of CD137 (4-1BB) and CD137L can supply a costimulatory signal to T cells through CD137. This costimulus seems to be more effective for CD8+ effector T cells than for CD4+ helper T cells (3). The interaction can also effect a reverse signal, inducing monocyte activation (2), or T cell inactivation and programmed death (4) through CD137L. Anti-CD137L antibody binds to CD137L present on Raji and U-937 cells, and also binds to recombinant CD137L-muCD8 in EIA. This antibody does not block the interaction between CD137 and CD137L.
Performance: Five x 105 cultured Raji human tumor cells were incubated 45 minutes on ice with 80 ul of anti-CD137L antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.29 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Group Header: anti-human CD137L
Immunogen: Recombinant human CD137L. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD137L are readily searchable from our website. Different antibodies against the same target such as CD137L may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.